Antithrombin III Polyclonal antibody

Antithrombin III Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16414-1-AP

Synonyms

Antithrombin, Antithrombin III, AT3, ATIII, Serpin C1, SERPINC1



Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16414-1-AP targets Antithrombin III in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Antithrombin III fusion protein Ag9565
Full Name serpin peptidase inhibitor, clade C (antithrombin), member 1
Calculated molecular weight 464 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC022309
Gene symbol SERPINC1
Gene ID (NCBI) 462
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

Protocols

Product Specific Protocols
WB protocol for Antithrombin III antibody 16414-1-APDownload protocol
IHC protocol for Antithrombin III antibody 16414-1-APDownload protocol
IF protocol for Antithrombin III antibody 16414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.

Authors - Takahiro Tougan