Tested Applications
| Positive WB detected in | human plasma, human brain tissue, human testis tissue, L02 cells, rat brain tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66052-1-Ig targets Serpin C1/Antithrombin III in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9880 Product name: Recombinant human SERPINC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-259 aa of BC022309 Sequence: MYSNVIGTVTSGKRKVYLLSLLLIGFWDCVTCHGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK Predict reactive species |
| Full Name | serpin peptidase inhibitor, clade C (antithrombin), member 1 |
| Calculated Molecular Weight | 464 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC022309 |
| Gene Symbol | Serpin C1/Antithrombin III |
| Gene ID (NCBI) | 462 |
| RRID | AB_11042589 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P01008 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Serpin C1/Antithrombin III antibody 66052-1-Ig | Download protocol |
| IHC protocol for Serpin C1/Antithrombin III antibody 66052-1-Ig | Download protocol |
| WB protocol for Serpin C1/Antithrombin III antibody 66052-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SERPINC1 (Antithrombin) antibody (Cat# 66052-1-Ig) has 4 citations. These appear in Matrix Biology, Orphanet Journal of Rare Diseases, Frontiers in Oncology, and Blood Vessel Thrombosis and Hemostasis. The associated research fields span human fertility/oncofertility biomarker discovery, severe thrombophilia (SERPINC1 variant characterization), lung cancer (PI3K/AKT pathway and anti-PD-1 therapy), and hemostasis/neutrophil regulation. Applications include IF and WB in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Orphanet J Rare Dis Clinical and functional characterization of p.Lys322stop variant in the SERPINC1 gene causing severe thrombophilia | ||
Front Oncol Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene | ||
Blood Vessel Thromb Hemost Antithrombin regulates neutrophil activities through the stimulation of C-type lectin family 1A. |
Reviews
What customers say
All four reviews rate this antibody 5 stars. Users report excellent performance in both Western Blot and IHC, with recommended dilutions of 1:1000–1:2000 for WB and 1:100–1:500 for IHC. Tested on colon cancer tissues and CRC cell lines, the antibody consistently produces clear, sharp bands. Reviewers describe it as a "very good antibody" that works well at the stated dilutions, with no issues or troubleshooting mentioned across any of the reviews.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Iram (Verified Customer) (03-23-2026) | Very good antibody
|
Iram (Verified Customer) (09-18-2025) | Very good antibody
|
Iram (Verified Customer) (01-15-2021) | Antithrombin III Monoclonal antibody giving a very clear sharp bands
|
Susmita (Verified Customer) (09-17-2020) | The antibody works good in mentioned dilution for WB and IHC
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