SERPINE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SERPINE2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 11303-1-AP

Synonyms

PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7

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Validation Data Gallery

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  WB analysis of C6 using 11303-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11303-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11303-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11303-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11303-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 11303-1-AP

  IP result of anti-SERPINE2 (IP:11303-1-AP, 3ug; Detection:11303-1-AP 1:300) with A549 cells lysate 2800ug.

• 

  IHC staining of human testis using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas cancer using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11303-1-AP (SERPINE2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, A549 cells, MCF-7 cells, rat brain tissue, rat heart tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human testis tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human testis tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Product Information

11303-1-AP targets SERPINE2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERPINE2 fusion protein Ag1830
• Full Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC015663
• Gene Symbol: SERPINE2
• Gene ID (NCBI): 5270
• RRID: AB_10732939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Publications

Species	Application	Title
human	WB,IHC,IP	Cancer Lett; SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.; Authors - Jingjing Zhang ; KD Validated
human	WB,IHC	Respir Res; High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma.; Authors - Ryota Dokuni ; KD Validated
human	WB	J Immunol; Deep Coverage Tissue and Cellular Proteomics Revealed IL-1β Can Independently Induce the Secretion of TNF-Associated Proteins from Human Synoviocytes.; Authors - Shengquan Tang
human	IP	Front Cell Dev Biol; BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma.; Authors - Xiyang Zhang
mouse	IF	Sci Rep; PMID: 30446696; Authors - Mary Ann Stepp
human	IHC	J Cell Physiol; Transcriptional profiling of endometriosis tissues identifies genes related to organogenesis defects.; Authors - Crispi Stefania S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ahmad (Verified Customer) (06-18-2021)

Fix in 1x PBS, 1% formaldehyde, 2 mM MgCl2, 5 mM EGTA, 0.02% NP-40 for 1 hr works good for staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: rabbit cornea

SERPINE2 Antibody Immunofluorescence validation (1:300 dilution) in rabbit cornea (Cat no:11303-1-AP)