Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells, C6 cells, MCF-7 cells, rat heart tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | mouse brain tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human testis tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11303-1-AP targets SERPINE2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1830 Product name: Recombinant human SERPINE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-397 aa of BC015663 Sequence: EELGSNTGIQVFNQIVKSRPHDNIVISPHGIASVLGMLQLGADGRTKKQLAMVMRYGVNGVGKILKKINKAIVSKKNKDIVTVANAVFVKNASEIEVPFVTRNKDVFQCEVRNVNFEDPASACDSINAWVKNETRDMIDNLLSPDLIDGVLTRLVLVNAVYFKGLWKSRFQPENTKKRTFVAADGKSYQVPMLAQLSVFRCGSTSAPNDLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHISTKTIDSWMSIMVPKRVQVILPKFTAVAQTDLKEPLKVLGITDMFDSSKANFAKITTGSENLHVSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFFIRHNPTGAVLFMGQINK Predict reactive species |
| Full Name | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44-50 kDa |
| GenBank Accession Number | BC015663 |
| Gene Symbol | SERPINE2 |
| Gene ID (NCBI) | 5270 |
| RRID | AB_10732939 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07093 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SERPINE2 antibody 11303-1-AP | Download protocol |
| IHC protocol for SERPINE2 antibody 11303-1-AP | Download protocol |
| IP protocol for SERPINE2 antibody 11303-1-AP | Download protocol |
| WB protocol for SERPINE2 antibody 11303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SERPINE2 Polyclonal antibody (Cat# 11303-1-AP) has 21 citations spanning journals including Autophagy, Advanced Science, Cancer Letters, Cell & Molecular Life Sciences, Journal of Biological Chemistry, Journal of Immunology, and Frontiers in Oncology. Research fields cover oncology (lung, gastric, colorectal, osteosarcoma, renal, and liver cancers), stroke and neuroinflammation, preeclampsia, aortic aneurysm, endometriosis, peripheral nerve injury, and radiation biology, with applications in WB, IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
Cell Mol Biol Lett Epigenetic modification regulates the ligamentum flavum hypertrophy through miR-335-3p/SERPINE2/β-catenin signaling pathway | ||
Cancer Lett SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.
| ||
Int J Biol Macromol Construction and validation of an osteosarcoma prognostic model reveals that SERPINE2 contributes to the progression and cisplatin sensitivity in osteosarcoma.
| ||
Comput Biol Med Microdissection of cancer-associated fibroblast infiltration subtypes unveils the secreted SERPINE2 contributing to immunosuppressive microenvironment and immuotherapeutic resistance in gastric cancer: A large-scale study integrating bulk and single-cell transcriptome profiling |
Reviews
What customers say
The single review for this product is a 5-star rating from an Immunofluorescence application on rabbit cornea tissue. The reviewer used a 1:300 dilution and reported that the antibody worked well for staining. They shared their fixation protocol: 1x PBS, 1% formaldehyde, 2 mM MgCl2, 5 mM EGTA, and 0.02% NP-40 for 1 hour. Overall, the feedback is positive, with the user confirming good staining performance under their described conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ahmad (Verified Customer) (06-18-2021) | Fix in 1x PBS, 1% formaldehyde, 2 mM MgCl2, 5 mM EGTA, 0.02% NP-40 for 1 hr works good for staining
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