SERTAD1 Polyclonal antibody

SERTAD1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 10167-1-AP

Synonyms

CDK4 binding protein p34SEI1, SEI 1, SEI1, SERTA domain containing 1, SERTAD1, TRIP Br1

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Validation Data Gallery

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  IHC staining of mouse heart using 10167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 10167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 10167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 10167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 10167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse heart tissue, mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10167-1-AP targets SERTAD1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERTAD1 fusion protein Ag0180
• Full Name: SERTA domain containing 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC002670
• Gene Symbol: SERTAD1
• Gene ID (NCBI): 29950
• RRID: AB_2285697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERTA domain containing 1 (SERTAD1, synonyms: SEI1, TRIP-Br1) protein is a cyclin-dependent kinase 4 (cdk4)-binding protein and acts as a growth factor sensor and facilitates the formation and activation of cyclin D-CDK complexes in the face of inhibitory levels of INK4 proteins, therefore, antagonizes the activity of p16(INK4a) tumor suppressor. SERTAD1 also suppresses CREB-mediated transcription and regulates cell cycle progression through sequential effects on the transcriptional activity of E2F-responsive promoters during G(1) and S phases.