SESN1 Recombinant antibody, PBS Only

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  WB analysis using 86008-1-RR (same clone as 86008-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86008-1-RR (SESN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86008-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86008-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86008-1-RR against Human SESN1 were performed. The affinity constant is 1.01 nM.

SESN1 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 86008-1-PBS

Clone No.250470G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:1.11.1.-, p53 regulated protein PA26, p53-regulated protein PA26, PA26, SEST1

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Product Information

86008-1-PBS targets SESN1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SESN1 fusion protein Ag16462
• Full Name: sestrin 1
• Calculated Molecular Weight: 551 aa, 64 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC112036
• Gene Symbol: SESN1
• Gene ID (NCBI): 27244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y6P5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).