• Featured Product
  • KD/KO Validated

Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 21346-1-AP

Synonyms

HI95, SES2, SESN2, SEST2, Sestrin 2, Sestrin2



Tested Applications

Positive WB detected inmouse thymus tissue, HeLa cells, K-562 cells, mouse lung tissue, NIH/3T3 cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21346-1-AP is Sestrin 2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag15669
Full Name sestrin 2
Calculated molecular weight 480 aa, 54 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberNM_031459
Gene symbol SESN2
Gene ID (NCBI) 83667
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Protocols

Product Specific Protocols
WB protocol for Sestrin 2 antibody 21346-1-APDownload protocol
IF protocol for Sestrin 2 antibody 21346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

MiR-4756 promotes albumin-induced renal tubular epithelial cell epithelial-to-mesenchymal transition and endoplasmic reticulum stress via targeting Sestrin2.

Authors - Yijie Jia
ratIF

Neurobiol Dis

Sestrin2 induced by hypoxia inducible factor1 alpha protects the blood-brain barrier via inhibiting VEGF after severe hypoxic-ischemic injury in neonatal rats.

Authors - Xudan Shi
ratIF

J Cereb Blood Flow Metab

Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups.

Authors - Xudan Shi
ratWB

Mol Cell Biol

Sestrin2 protects dopaminergic cells against rotenone toxicity through AMPK-dependent autophagy activation.

Authors - Yi-Sheng Hou
humanWB,IF

J Invest Dermatol

Metastatic melanoma cells rely on Sestrin2 to acquire anoikis resistance via detoxifying intracellular ROS.

Authors - Guannan Zhu
  • KD Validated
humanWB

Elife

Dynamics of mTORC1 activation in response to amino acids.

Authors - Maria Manifava
  • KD Validated