|Positive WB detected in
|mouse thymus tissue, HeLa cells, K-562 cells, mouse lung tissue, NIH/3T3 cells
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 21346-1-AP is Sestrin 2 Fusion Protein expressed in E. coli.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Sestrin 2 fusion protein Ag15669
|Calculated molecular weight
|480 aa, 54 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).
p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.
Dynamics of mTORC1 activation in response to amino acids.
Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage
J Invest Dermatol
Metastatic melanoma cells rely on Sestrin2 to acquire anoikis resistance via detoxifying intracellular ROS.
Mol Cell Biol
Sestrin2 protects dopaminergic cells against rotenone toxicity through AMPK-dependent autophagy activation.
J Cereb Blood Flow Metab
Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups.