SESN3 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84823-3-RR (same clone as 84823-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84823-3-RR (SESN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84823-3-RR (same clone as 84823-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84823-3-RR (SESN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84823-3-RR (same clone as 84823-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SESN3 antibody (84823-3-RR, Clone: 242189D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84823-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84823-3-RR against Human SESN3 were performed. The affinity constant is 6.78 nM.

SESN3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84823-3-PBS

Clone No.242189D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242189D1, EC:1.11.1.-, SEST3, sestrin 3, Sestrin-3

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Product Information

84823-3-PBS targets SESN3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag17872

  Product name: Recombinant human SESN3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-321 aa of BC017296

  Sequence: MNRGGGSPSAAANYLLCTNCRKVLRKDKRIRVSQPLTRGPSAFIPEKEVVQANTVDERTNFLVEEYSTSGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLINMHVDEFLKTGGIAEWLNGLEYVPQRLKNLNEINKLLAHRPWLITKEHIQKLVKTGENNWSLPELVHAVVLLAHYHALASFVFGSGINPERDPEISNGFRLISVNNFCVCDLANDNNIENASLSGSNFGIVDSLSELEALMERMKRLQEEREDEEASQEEMSTRFEKEKKESLFVVSGDTFHSFPHSGAFLHFFAF

• Full Name: sestrin 3
• Calculated Molecular Weight: 492 aa, 57 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: BC017296
• Gene Symbol: SESN3
• Gene ID (NCBI): 143686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P58005
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT-induced increase in ROS. It might be a promising target in selectively killing cancer cells with high AKT activity levels.