SOLO/SESTD1 Polyclonal antibody

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  WB analysis using 23911-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23911-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO/SESTD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 23911-1-AP

  IP result of anti-SOLO (IP:23911-1-AP, 4ug; Detection:23911-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of rat brain using 23911-1-AP

  IP result of anti-SOLO (IP:23911-1-AP, 0ug; Detection:23911-1-AP 1:4000) with rat brain tissue lysate 1120 ug.

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  IF Staining of HepG2 using 23911-1-AP

  Immunofluorescent analysis of HepG2 cells using 23911-1-AP (SOLO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Jurkat using 23911-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SOLO antibody (23911-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

SOLO/SESTD1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 23911-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

SESTD1, SOLO, Huntingtin-interacting protein-like protein, Protein Solo, SEC14 and spectrin domains 1

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue, L02 cells
• Positive IP detected in: mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: Jurkat cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23911-1-AP targets SOLO/SESTD1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21016

  Product name: Recombinant human SESTD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 348-696 aa of BC047578

  Sequence: DLVELKSLQQQLSDVCYRQASQLEFRQNLLQAALEFHGVAQDLSQQLDGLLGMLCVDVAPADGASIQQTLKLLEEKLKSVDVGLQGLREKGQGLLDQISNQASWAYGKDVTIENKENVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDAAQAVEWLSELLDALLKTHIRLGDDAQETKVLLEKHRKFVDVAQSTYDYGRQLLQATVVLCQSLRCTSRSSGDTLPRLNRVWKQFTIASEERVHRLEMAIAFHSNAEKILQDCPEEPEAINDEEQFDEIEAVGKSLLDRLTVPVVYPDGTEQYFGSPSDMASTAENIRDRMKLVNLKRQQLRHPEMVTTES

• Full Name: SEC14 and spectrin domains 1
• Calculated Molecular Weight: 696 aa, 79 kDa
• Observed Molecular Weight: 70-79 kDa
• GenBank Accession Number: BC047578
• Gene Symbol: SOLO
• Gene ID (NCBI): 91404
• RRID: AB_2879360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q86VW0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC14 domain and spectrin repeat-containing protein 1 (SESTD1) is also named Huntingtin-interacting protein-like protein and SOLO. SESTD1, a previously uncharacterized lipid-binding protein strongly expressed in aorta, was found to interact with both TRPC proteins and modulate receptor-activated TRPC5 currents in a recombinant cell line (PMID: 20164195). SESTD1 is proposed to be involved in phospholipid regulation of transient receptor potential channels TRPC4 and TRPC5 (PMID: 26503763). SESTD1 expression is associated with high abundance of immune infiltration, including T helper cells, Th2 cells, and Macrophages in HCC (PMID: 41016345).