SET Monoclonal antibody

SET Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2B4D1

Reviews (1)

Cat no : 67478-1-Ig

Synonyms

2PP2A, HLA DR associated protein II, I 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET, SET, SET nuclear oncogene, TAF I, TAF IBETA, Template activating factor I

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Validation Data Gallery

• 

  WB analysis of Jurkat using 67478-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67478-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67478-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67478-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67478-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, 4T1 cells, HeLa cells, HepG2 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67478-1-Ig targets SET in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SET nuclear oncogene
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: NM_003011
• Gene Symbol: SET
• Gene ID (NCBI): 6418
• RRID: AB_2882705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell proliferation via many pathways that promote tumorigenesis and metastasis.

Publications

Species	Application	Title
human	WB	Cell Rep; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression; Authors - Ling Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chantal (Verified Customer) (10-04-2022)

Specific band appeared after 3 minutes of exposure. it works on mouse and human cell lines

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MEFs, Hela, HEK293FT

SET Antibody Western Blot validation (1:1000 dilution) in MEFs, Hela, HEK293FT (Cat no:67478-1-Ig)