SETX Polyclonal antibody

• 

  WB analysis using 13837-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13837-1-AP (SETX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 13837-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13837-1-AP (SETX antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SETX Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13837-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Senataxin, SCAR1, KIAA0625, EC:3.6.4.-, AOA2

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13837-1-AP targets SETX in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4785

  Product name: Recombinant human SETX protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 575-930 aa of BC032600

  Sequence: LVMEIIKLIKDKRKDVSFRNIGIITHYKAQKTMIQKDLDKEFDRKGPAEVDTVDAFQGRQKDCVIVTCVRANSIQGSIGFLASLQRLNVTITRAKYSLFILGHLRTLMENQHWNQLIQDAQKRGAIIKTCDKNYRHDAVKILKLKPVLQRSLTHPPTIAPEGSRPQGGLPSSKLDSGFAKTSVAASLYHTPSDSKEITLTVTSKDPERPPVHDQLQDPRLLKRMGIEVKGGIFLWDPQPSSPQHPGATPPTGEPGFPVVHQDLSHIQQPAAVVAALSSHKPPVRGEPPAASPEASTCQSKCDDPEEELCHRREARAFSEGEQEKCGSETHHTRRNSRWDKRTLEQEDSSSKKRKLL

• Full Name: senataxin
• Calculated Molecular Weight: 2677 aa, 303 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: BC032600
• Gene Symbol: SETX
• Gene ID (NCBI): 23064
• RRID: AB_3085425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z333
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETX is involved in DNA double-strand breaks damage response generated by oxidative stress (PMID:17562789). It might also plays a role in the development and maturation of germ cells.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Biochem Biophys Res Commun; TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia.; Authors - Sarina Han