SF3A1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• SF3A1 Polyclonal antibody (15858-1-AP)

SF3A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15858-1-AP Copied!

Publications (11)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Spliceosome-associated protein 114, SF3a120, SAP114, SAP 114, PRP21

• 

  WB analysis using 15858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 15858-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HL-60 using 15858-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 15858-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15858-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse lung using 15858-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 15858-1-AP

  IP result of anti-SF3A1 (IP:15858-1-AP, 4ug; Detection:15858-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15858-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15858-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15858-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15858-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15858-1-AP

  Immunofluorescent analysis of HepG2 cells, using SF3A1 antibody 15858-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SF3A1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HL-60 cells, mouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, K-562 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15858-1-AP targets SF3A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8196

  Product name: Recombinant human SF3A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 443-793 aa of BC007684

  Sequence: DRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPPSSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVPMPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSLAYYNMANGAVIHLALKERGGRKK

• Full Name: splicing factor 3a, subunit 1, 120kDa
• Calculated Molecular Weight: 793 aa, 89 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC007684
• Gene Symbol: SF3A1
• Gene ID (NCBI): 10291
• RRID: AB_2186354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15459
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )

Publications

What published studies show

The anti-SF3A1 antibody (Cat# 15858-1-AP) has 11 citations published in journals including Molecular Cell, Oncogene, Cell Reports, Nature Communications, Journal of Translational Medicine, European Journal of Pharmacology, Frontiers in Pharmacology, and iScience. Research fields span RNA splicing regulation, cancer (ovarian, prostate, renal cell carcinoma), bone metastasis, mitochondrial energy metabolism, inflammation, and non-coding RNA biology. Applications include WB, IHC, and IP in human and mouse samples.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; ZC3H14 facilitates backsplicing by binding to exon-intron boundary and 3' UTR; Authors - Qiqi Li
mouse	WB	J Transl Med; Pharmacological targeting of SF3a1-MDM4 splicing vulnerability in ovarian cancer with rifaximin and antisense oligonucleotide.; Authors - Xueqian Shuai ; KD Validated
human	IHC	Oncogene; The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC; Authors - Yiqiu Wang
human	WB	Cell Rep; SF3B1 Association with Chromatin Determines Splicing Outcomes.; Authors - Nir Kfir
		Cell Rep; Integrative identification of non-coding regulatory regions driving metastatic prostate cancer; Authors - Brian J Woo
human	WB	Eur J Pharmacol; Hydroxysafflor yellow A alleviates ischemic myocardial injury by targeting SF3A1 to improve mitochondrial energy metabolism; Authors - Bo-Kai Wang