SF3A1 Polyclonal antibody

SF3A1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15858-1-AP

Synonyms

PRP21, PRPF21, SAP 114, SAP114, SF3A1, SF3A120, Splicing factor 3A subunit 1



Tested Applications

Positive WB detected inHL-60 cells, HEK-293 cells, mouse lung tissue, mouse brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15858-1-AP targets SF3A1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF3A1 fusion protein Ag8196
Full Name splicing factor 3a, subunit 1, 120kDa
Calculated molecular weight 793 aa, 89 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC007684
Gene symbol SF3A1
Gene ID (NCBI) 10291
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )

Protocols

Product Specific Protocols
WB protocol for SF3A1 antibody 15858-1-APDownload protocol
IHC protocol for SF3A1 antibody 15858-1-APDownload protocol
IF protocol for SF3A1 antibody 15858-1-APDownload protocol
IP protocol for SF3A1 antibody 15858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

SF3B1 Association with Chromatin Determines Splicing Outcomes.

Authors - Nir Kfir