SF3A1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86170-1-RR (same clone as 86170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86170-1-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86170-1-PBS in a different storage buffer formulation.

SF3A1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86170-1-PBS

Clone No.250768E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114

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Product Information

86170-1-PBS targets SF3A1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8196

  Product name: Recombinant human SF3A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 443-793 aa of BC007684

  Sequence: DRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPPSSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVPMPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSLAYYNMANGAVIHLALKERGGRKK

• Full Name: splicing factor 3a, subunit 1, 120kDa
• Calculated Molecular Weight: 793 aa, 89 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC007684
• Gene Symbol: SF3A1
• Gene ID (NCBI): 10291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15459
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )