SF3A3 Polyclonal antibody

SF3A3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12070-1-AP

Synonyms

PRP9, PRPF9, SAP 61, SAP61, SF3A3, SF3a60, Splicing factor 3A subunit 3



Tested Applications

Positive WB detected inA431 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12070-1-AP targets SF3A3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF3A3 fusion protein Ag2708
Full Name splicing factor 3a, subunit 3, 60kDa
Calculated molecular weight 501 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC011523
Gene symbol SF3A3
Gene ID (NCBI) 10946
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SF3A3 antibody 12070-1-APDownload protocol
IHC protocol for SF3A3 antibody 12070-1-APDownload protocol
IF protocol for SF3A3 antibody 12070-1-APDownload protocol
IP protocol for SF3A3 antibody 12070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols