SF3B1 Polyclonal antibody

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SF3B1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27684-1-AP Copied!

Publications (13)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, CoIP, ELISA

Synonyms

PRP10, SAP 155, SAP155, SF3b155

• 

  WB analysis of MCF-7 using 27684-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 27684-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U2OS using 27684-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 27684-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 27684-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27684-1-AP (SF3B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 27684-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27684-1-AP (SF3B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, U2OS cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27684-1-AP targets SF3B1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26689

  Product name: Recombinant human SF3B1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-126 aa of NM_012433

  Sequence: MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTM

• Full Name: splicing factor 3b, subunit 1, 155kDa
• Calculated Molecular Weight: 146 kDa
• Observed Molecular Weight: 146-150 kDa
• GenBank Accession Number: NM_012433
• Gene Symbol: SF3B1
• Gene ID (NCBI): 23451
• RRID: AB_2880946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75533
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Publications

What published studies show

SF3B1 Polyclonal antibody (Cat# 27684-1-AP) has 13 citations published in journals including Nature Communications, Molecular Cell, Gastroenterology, Science Advances, Genome Medicine, EMBO Journal, PLoS Biology, Cancer Gene Therapy, EMBO Reports, Scientific Reports, and Journal of Biological Chemistry. Research fields span RNA splicing, cancer biology, stem cell differentiation, antiviral immunity, DNA damage response, myelodysplastic syndrome, and progeria.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
human	WB	Nat Commun; Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12; Authors - Zixiang Wang
human	WB	Mol Cell; RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeria.; Authors - Amit K. Behera
human	WB	Mol Cell; Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles; Authors - Wael Kamel
human	WB	Sci Adv; Nuclear accumulation of YTHDF1 regulates mRNA splicing in the DNA damage response; Authors - Jingyu Hou
human	WB	Genome Med; A novel spliceosomopathy caused by de novo SF3B3 variants.; Authors - Luciana Musante

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

One reviewer (Wael K.) rated this product 5/5 stars for Western Blot on HEK293 cells at a 1:2000 dilution (Lot 00055418). They reported a specific band between 100 and 150 kDa, indicating good specificity and performance under their conditions.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Wael (Verified Customer) (09-29-2022)

specific band between 100 and 150 kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hek293

SF3B1 Antibody Western Blot validation (1:2000 dilution) in Hek293 (Cat no:27684-1-AP)