SF3B2 Polyclonal antibody

SF3B2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10919-1-AP

Synonyms

Spliceosome-associated protein 145, SF3b150, SF3b145, SAP145, SAP 145

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Validation Data Gallery

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  WB analysis using 10919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10919-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10919-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10919-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10919-1-AP (SF3B2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10919-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10919-1-AP (SF3B2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HEK-293 using 10919-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SF3B2 antibody (10919-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

10919-1-AP targets SF3B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SF3B2 fusion protein Ag1009
• Full Name: splicing factor 3b, subunit 2, 145kDa
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC007610
• Gene Symbol: SF3B2
• Gene ID (NCBI): 10992
• RRID: AB_2285782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3B (splicing factor 3B) is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B complex contains the spliceosome-associated protein (SAP) 49, 130, 145, and 155 has been demonstrated. And this complex is essential for U2 snRNP to anchor to the pre-mRNA. Splicing factor 3B subunit 2 (SF3B2), known as SAP145, forms complex with SAP49 which plays a role in cell cycle progression. SAP145, with predicated MW of 100 kDa, appears with MW of about 145 kDa which may be due to the post-translational modification.

Publications

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Nucleic Acids Res; CUGBP2 directly interacts with U2 17S snRNP components and promotes U2 snRNA binding to cardiac troponin T pre-mRNA.; Authors - Goo Young-Hwa YH
human	WB	Front Oncol; Novel Non-Coding Transcript in NR4A3 Locus, LncNR4A3, Regulates RNA Processing Machinery Proteins and NR4A3 Expression.; Authors - Ada Congrains
human	WB	J Exp Clin Cancer Res; CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling.; Authors - Dongni Chen
human	IHC	Cell Rep; Spatial region-resolved proteome map reveals mechanism of COVID-19-associated heart injury.; Authors - Ling Leng