SF3B4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SF3B4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 10482-1-AP

Synonyms

SAP 49, SAP49, SF3B4, SF3b49, SF3b50, Splicing factor 3B subunit 4

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Validation Data Gallery

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  WB analysis of HEK-293T using 10482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 10482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10482-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10482-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10482-1-AP

  IP result of anti-SF3B4 (IP:10482-1-AP, 4ug; Detection:10482-1-AP 1:500) with HeLa cells lysate 1600ug.

• 

  IHC staining of human pancreas cancer using 10482-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10482-1-AP (SF3B4 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10482-1-AP targets SF3B4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SF3B4 fusion protein Ag0737
• Full Name: splicing factor 3b, subunit 4, 49kDa
• Calculated Molecular Weight: 44 aa, 3 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004273
• Gene Symbol: SF3B4
• Gene ID (NCBI): 10262
• RRID: AB_2301639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3B4, also named as Pre-mRNA-splicing factor SF3b 49 kDa subunit, is a 424 amino acid protein, which belongs to the SF3B4 family. SF3B4 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. The calculated molecular weight of SF3B4 is 44 kDa. Based on its predicted molecular mass and the observed migration of the 50kDa cross-linked species on two-dimensional gels.(PMID: 9614130)

Publications

Species	Application	Title
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human,rat	WB	J Mol Cell Cardiol; Remodeling of the m6A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy.; Authors - Scott A Hinger
human	WB,IHC	Cell Death Dis; SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.; Authors - Yuchao Diao ; KD Validated
	WB	bioRxiv; Sf3b4 mutation in Xenopus tropicalis causes RNA splicing defects followed by massive gene dysregulation that disrupt cranial neural crest development; Authors - Casey Griffin