SF3B5 Polyclonal antibody

SF3B5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15525-1-AP

Synonyms

Splicing factor 3B subunit 5, SF3B10, Pre-mRNA-splicing factor SF3b 10 kDa subunit

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 15525-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15525-1-AP (SF3B5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15525-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15525-1-AP (SF3B5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 15525-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 15525-1-AP (SF3B5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 15525-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15525-1-AP (SF3B5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15525-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15525-1-AP (SF3B5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15525-1-AP

  Immunofluorescent analysis of Hela cells, using SF3B5 antibody 15525-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, mouse eye tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15525-1-AP targets SF3B5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SF3B5 fusion protein Ag7205
• Full Name: splicing factor 3b, subunit 5, 10kDa
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10-12 kDa
• GenBank Accession Number: BC000198
• Gene Symbol: SF3B5
• Gene ID (NCBI): 83443
• RRID: AB_10596628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.