SFMBT2 Polyclonal antibody

SFMBT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25256-1-AP

Synonyms

KIAA1617, SFMBT2

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Validation Data Gallery

• 

  WB analysis using 25256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 25256-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 25256-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells, mouse spleen tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

25256-1-AP targets SFMBT2 in WB, CoIP, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFMBT2 fusion protein Ag19379
• Full Name: Scm-like with four mbt domains 2
• Calculated Molecular Weight: 894 aa, 101 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC152430
• Gene Symbol: SFMBT2
• Gene ID (NCBI): 57713
• RRID: AB_2833000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFMBT2 (Scm like with four mbt domains 2), also named as KIAA1617. It is located in Nucleus and ubiquitous expression in thyroid and ovary. The calculated molecular weight of SFMBT2 is 100 kDa. Although mammalian SFMBTs have similar structural features with PcG protein dSFMBT, biological function and target genes of SFMBT have been not investigated well. Lee K and Na W investigated biological function of human SFMBT2 that interacted with YY1 using AR (androgen receptor)-negative DU145 prostate cancer cells. They found that human SFMBT2 represses HOXB13 gene expression via its association with methylated histones H3 and H4 that are transcriptional repression marks. Furthermore, their findings indicate that SFMBT2 may be involved in regulation of cell growth of DU145 prostate cancer cells (PMID: 23385818).

Publications

Species	Application	Title
mouse		Cell Stem Cell; Overcoming Intrinsic H3K27me3 Imprinting Barriers Improves Post-implantation Development after Somatic Cell Nuclear Transfer.; Authors - Le-Yun Wang
		Int J Mol Med; SFMBT2 positively regulates SOX9 and chondrocyte proliferation.; Authors - Safdar Hussain
human	WB,CoIP	Biol Direct; Acetylation- and ubiquitination-regulated SFMBT2 acts as a tumor suppressor in clear cell renal cell carcinoma; Authors - Qingpeng Xie