Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human skin cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15328-1-AP targets SFRP4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6909 Product name: Recombinant human SFRP4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-346 aa of BC058911 Sequence: MFLSILVALCLWLHLALGVRGAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDVDCKRLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAPKPASPKKNIKTRSAQKRTNPKRV Predict reactive species |
| Full Name | secreted frizzled-related protein 4 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 41-51 kDa, 55 kDa |
| GenBank Accession Number | BC058911 |
| Gene Symbol | SFRP4 |
| Gene ID (NCBI) | 6424 |
| RRID | AB_2187099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6FHJ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| IP protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| WB protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SFRP4 antibody (Cat# 15328-1-AP) has 21 citations published in journals including Nature Communications, Clinical and Translational Medicine, Journal of Investigative Dermatology, Life Sciences, Gene Therapy, European Journal of Pharmacology, Chemical & Biological Interactions, Scientific Reports, Regenerative Therapy, Endocrinology, Pathology Research & Practice, Molecular and Cellular Endocrinology, Adipocyte, American Journal of Cancer Research, Cell Physiology & Biochemistry, Pharmacogenomics and Personalized Medicine, Journal of Oncology, Experimental and Therapeutic Medicine, and Journal of Gastrointestinal Oncology. Research fields span Wnt signaling, cancer biology, fibrosis, adipogenesis, stroke, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Myofibroblast transcriptome indicates SFRP2hi fibroblast progenitors in systemic sclerosis skin. | ||
Clin Transl Med Spatial transcriptomics reveals prognosis-associated cellular heterogeneity in the papillary thyroid carcinoma microenvironment | ||
J Invest Dermatol Fibroblast subpopulations in Systemic Sclerosis - Functional implications of individual subpopulations and correlations with clinical features | ||
Life Sci Short peptide domains of the Wnt inhibitor sFRP4 target ovarian cancer stem cells by neutralizing the Wnt β-catenin pathway, disrupting the interaction between β-catenin and CD24 and suppressing autophagy | ||
Gene Ther Effect of nanoparticle-mediated delivery of SFRP4 siRNA for treating Dupuytren disease.
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