SFRS17A Polyclonal antibody

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  WB analysis of HeLa using 13441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13441-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 13441-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13441-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13441-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 13441-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13441-1-AP (SFRS17A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13441-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13441-1-AP (SFRS17A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse cerebellum using 13441-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13441-1-AP (SFRS17A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 13441-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13441-1-AP (SFRS17A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-1080 using 13441-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using SFRS17A antibody (13441-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SFRS17A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13441-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CXYorf3, B-lymphocyte antigen, A-kinase anchor protein 17A, AKAP-17A, AKAP17A

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, human heart tissue, human kidney tissue, HEK-293T cells
• Positive IHC detected in: mouse cerebellum tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HT-1080 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13441-1-AP targets SFRS17A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4252

  Product name: Recombinant human SFRS17A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC028151

  Sequence: MAAATIVHDTSEAVELCPAYGLYLKPITKMTISVALPQLKQPGKSISNWEVMERLKGMVQNHQFSTLRISKSTMDFIRFEGEVENKSLVKSFLACLDGKTIKLSGFSDILKVRAAEFKIDFPTRHDWDSFFRDAKDMNETLPGERPDTIHLEGLPCKWFALKESGSEKPSEDVLVKVFEKFGEIRNVDIPMLDSYREEMTGRNFHTFSFGGHLNFEAYVQYREYMGFIQAMSALRGMKLMYKGEDGKAVACNIKVSFDSTKHLSDASIKKRQLERQKLQELEQQREEQKRREKEAEERQRAEERKQKELEELERERKREEKLRKREQKQRDRELRRNQKKLEKLQAEEQK

• Full Name: splicing factor, arginine/serine-rich 17A
• Calculated Molecular Weight: 695 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC028151
• Gene Symbol: SFRS17A
• Gene ID (NCBI): 8227
• RRID: AB_2184937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02040
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.