SFRS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SFRS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (2)

Cat no : 20371-1-AP

Synonyms

Serine/arginine-rich splicing factor 2, SC-35, SC35, SC 35, Protein PR264

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Validation Data Gallery

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  WB analysis using 20371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 20371-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20371-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20371-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20371-1-AP

  IP result of anti-SFRS2 (IP:20371-1-AP, 4ug; Detection:20371-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 20371-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20371-1-AP (SFRS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20371-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20371-1-AP (SFRS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20371-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS2 antibody (20371-1-AP) at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse testis tissue, A549 cells, rat brain tissue, HepG2 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 3 publications below

Product Information

20371-1-AP targets SFRS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: splicing factor, arginine/serine-rich 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-35 kDa
• GenBank Accession Number: NM_003016
• Gene Symbol: SFRS2
• Gene ID (NCBI): 6427
• RRID: AB_2616311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS2, also named as PR264 and Splicing component, 35 kDa, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. The molecular weight of modified SFRS2 is 35-40kDa. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.

Publications

Species	Application	Title
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	EMBO Mol Med; SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity; Authors - Indranil Malik
human,mouse	WB	Am J Cancer Res; tRF-20-M0NK5Y93-induced MALAT1 promotes colon cancer metastasis through alternative splicing of SMC1A; Authors - Na Luan ; KD Validated
human	WB	Neuroscience; The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain.; Authors - Obayed Raihan
mouse	WB	Cell Rep; SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.; Authors - Obayed Raihan
human	WB	Anal Chem; Amyotrophic Lateral Sclerosis-Associated Mutants of SOD1 Perturb mRNA Splicing through Aberrant Interactions with SRSF2; Authors - Xingyuan Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xingyuan (Verified Customer) (08-15-2023)

Very nice quality at 1:500 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: hek293t

SFRS2 Antibody Western Blot validation (1:500 dilution) in hek293t (Cat no:20371-1-AP)

FH

Xingyuan (Verified Customer) (07-17-2023)

The quality of SRSF2's antibody wasn't good. I was not able to see bands at around 37kDa (tried 3 times).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

SFRS2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:20371-1-AP)