SFRS5 Polyclonal antibody

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• SFRS5 Polyclonal antibody (16237-1-AP)

SFRS5 Polyclonal Antibody for WB, ELISA

Cat No. 16237-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

Delayed early protein HRS, HRS, Pre mRNA splicing factor SRP40, SFRS5, SRP40, SRSF5

• 

  WB analysis of mouse testis using 16237-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16237-1-AP (SFRS5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 16237-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16237-1-AP (SFRS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16237-1-AP targets SFRS5 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8988

  Product name: Recombinant human SFRS5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-272 aa of BC018823

  Sequence: MSGCRVFIGRLNPAAREKDVERFFKGYGRIRDIDLKRGFGFVEFEDPRDADDAVYELDGKELCSERVTIEHARARSRGGRGRGRYSDRFSSRRPRNDRRNAPPVRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASYGDLKNAIEKLSGKEINGRKIKLIEGSKRHSRSRSRSRSRTRSSSRSRSRSRSRSRKSYSRSRSRSRSRSRSKSRSVSRSPVPEKSQKRGSSSRSKSPASVDRQRSRSRSRSRSVDSGN

• Full Name: splicing factor, arginine/serine-rich 5
• Calculated Molecular Weight: 272 aa, 31 kDa
• GenBank Accession Number: BC018823
• Gene Symbol: SFRS5
• Gene ID (NCBI): 6430
• RRID: AB_2185204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13243
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SR proteins own a characteristic C-terminal Ser/Arg-rich repeat (RS domain) of variable length and constitute a family of highly conserved nuclear phosphoproteins that can function as both essential and alternative pre-mRNA splicing factors [PMID:7556075]. The binding of SR proteins to pre-mRNA appears to be one of the earliest steps in spliceosome assembly, enhancing the binding of U1 snRNP to the 5' splice site to form a commitment complex [PMID:1577277 ]. Alternative splicing has a major role in the regulation of gene expression. SFRS5 belongs to the serine/arginine (SR) protein family of regulators of alternative pre-mRNA splicing [PMID:9244433].

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	WB	JCI Insight; Splicing factor SRSF6 mediates pleural fibrosis.; Authors - Li-Mei Liang
human	IF	Molecules; L-Arginine Enhances Oral Keratinocyte Proliferation under High-Glucose Conditions via Upregulation of CYP1A1, SKP2, and SRSF5; Authors - Junhe Shi
mouse		J Dent Res; Mutations Causing X-Linked Amelogenesis Imperfecta Alter miRNA Formation from Amelogenin Exon4; Authors - R Shemirani
mouse	WB	Cell Rep; Aspartyl-tRNA synthetase 2 orchestrates iron-sulfur metabolism in hematopoietic stem cells via fine-tuning alternative RNA splicing; Authors - Xuan Gu

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Yan (Verified Customer) (04-28-2021)

Signals was observed in the nucleus when I performed immunofluorescence.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Osteosarcoma Epithelial Cells