SFRS9 Polyclonal antibody

• Featured Product
• KD/KO Validated

SFRS9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 17926-1-AP

Synonyms

SRSF9, SRP30C, Splicing factor, arginine/serine-rich 9, Serine/arginine-rich splicing factor 9, Pre-mRNA-splicing factor SRp30C

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Validation Data Gallery

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  WB analysis of HEK-293 using 17926-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17926-1-AP (SFRS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17926-1-AP (SFRS9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: mouse kidney tissue, human cervical cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

17926-1-AP targets SFRS9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRS9 fusion protein Ag12347
• Full Name: splicing factor, arginine/serine-rich 9
• Calculated Molecular Weight: 221 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC093973
• Gene Symbol: SFRS9
• Gene ID (NCBI): 8683
• RRID: AB_2270087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS9 is a member of the serine/arginine (SR) splicing factor family, which contains an N-terminal RNA recognition motif (RRM), a glycine-rich region, an internal region homologous to the RRM, and a long (315-amino-acid) C-terminal domain composed predominantly of alternating serine and arginine residues. SRSF9 has been linked to the occurrence and progression of hepatocellular carcinoma (HCC), colorectal cancer (CRC) and oral squamous cell carcinoma (OSCC). In HCC and CRC, the knockdown of SRSF9 inhibited the proliferation and migration of cancer cells, cell cycle progression and colony formation of cancer cells (PMID: 34336668,35971121).

Publications

Species	Application	Title
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan
human	WB	J Cell Biol; Stress granules regulate stress-induced paraspeckle assembly.; Authors - Haiyan An
human	WB	Hum Mutat; Antisense oligonucleotides modulate dopa decarboxylase function in aromatic l-amino acid decarboxylase deficiency.; Authors - Chi-Ren Tsai
human	WB	J Cancer Res Clin Oncol; SRSF9 promotes cell proliferation and migration of glioblastoma through enhancing CDK1 expression; Authors - Chunyuan Luo
human	WB	Cell Death Dis; Chemotoxicity-induced exosomal lncFERO regulates ferroptosis and stemness in gastric cancer stem cells.; Authors - Haiyang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jingwen (Verified Customer) (04-28-2021)

The staining signal showed up inside the nucleus.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Human cancer cell line