SFT2D3 Polyclonal antibody

SFT2D3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19758-1-AP

Synonyms

SFT2 domain containing 3, SFT2D3

---

Validation Data Gallery

• 

  WB analysis of HeLa using 19758-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19758-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19758-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19758-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 19758-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 19758-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 19758-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19758-1-AP (SFT2D3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 19758-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19758-1-AP (SFT2D3 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human liver tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19758-1-AP targets SFT2D3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SFT2 domain containing 3
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: NM_032740
• Gene Symbol: SFT2D3
• Gene ID (NCBI): 84826
• RRID: AB_10640432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFT2D3 belongs to the SFT2 family. SFT2D3 may be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.