SFXN3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SFXN3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15156-1-AP

Synonyms

SFX3, SFXN3, sideroflexin 3

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Validation Data Gallery

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  WB analysis of HeLa using 15156-1-AP

  WB result of SFXN3 antibody (15156-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN3 transfected HeLa cells.

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  WB analysis using 15156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15156-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 15156-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 15156-1-AP (SFXN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, Neuro-2a cells, rat brain tissue, NIH/3T3 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

15156-1-AP targets SFXN3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFXN3 fusion protein Ag7203
• Full Name: sideroflexin 3
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000124
• Gene Symbol: SFXN3
• Gene ID (NCBI): 81855
• RRID: AB_2185825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFXN3, a mitochondrial membrane protein, is a member of sideroflexin family. Serum anti-SFXN3 autoantibody was elevated in early stage of the oral squamous cell carcinoma significantly, indicating its clinical value for diagnosis of oral squamous cell carcinoma.

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.; Authors - Mariluz Soula
human	IHC	PeerJ; Identification of iron metabolism-related genes as prognostic indicators for papillary thyroid carcinoma: a retrospective study; Authors - Tiefeng Jin
human,mouse	WB	Nucleic Acids Res; Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription; Authors - Jingke Xie
human	WB	Am J Respir Crit Care Med; Distinct Cancer-Promoting Stromal Gene Expression Depending on Lung Function.; Authors - Brian J Sandri