Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11936-1-AP targets Beta Sarcoglycan in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Beta Sarcoglycan fusion protein Ag2533 Predict reactive species |
| Full Name | sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) |
| Calculated Molecular Weight | 318 aa, 35 kDa |
| Observed Molecular Weight | 45-48 kDa |
| GenBank Accession Number | BC020709 |
| Gene Symbol | Beta Sarcoglycan |
| Gene ID (NCBI) | 6443 |
| RRID | AB_10699870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16585 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SGCB is a 43-kD component of the dystrophin-glycoprotein complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Sarcoglycan antibody 11936-1-AP | Download protocol |
| IHC protocol for Beta Sarcoglycan antibody 11936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Cardioprotective effect of icariin against myocardial fibrosis and its molecular mechanism in diabetic cardiomyopathy based on network pharmacology: Role of ICA in DCM. | ||
Acta Histochem Exploring the oncogenic role of RGS19 in bladder cancer progression and prognosis | ||
Oncotarget Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro. | ||
J Pharm Biomed Anal Study on the mechanism of Xinmailong injection against chronic heart failure based on transcriptomics and proteomics | ||
Pain Res Manag Exploring the Analgesic Initiation Mechanism of Tuina in the Dorsal Root Ganglion of Minor CCI Rats via the TRPV1/TRPA1-cGMP Pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shushu (Verified Customer) (07-24-2025) | The antibody appears to work for the most part, as bands of the expected size are present in most of the positive samples. However, there are also numerous nonspecific bands of varying sizes. Optimization is needed.
 |
FH Venkata (Verified Customer) (01-03-2022) | I have loaded 50 and 100ug of protein in each of the lanes and the samples are from mouse heart and skeletal muscle tissues. I don't see expected bands in all the samples and most of the bands are non specific.
 |
