Beta Sarcoglycan Polyclonal antibody

Beta Sarcoglycan Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 11936-1-AP

Synonyms

43DAG, A3b, Beta sarcoglycan, Beta SG, LGMD2E, SGC, SGCB

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 11936-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 11936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

11936-1-AP targets Beta Sarcoglycan in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Sarcoglycan fusion protein Ag2533
• Full Name: sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
• Calculated Molecular Weight: 318 aa, 35 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC020709
• Gene Symbol: Beta Sarcoglycan
• Gene ID (NCBI): 6443
• RRID: AB_10699870
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SGCB is a 43-kD component of the dystrophin-glycoprotein complex.

Publications

Species	Application	Title
rat	WB	Phytomedicine; Cardioprotective effect of icariin against myocardial fibrosis and its molecular mechanism in diabetic cardiomyopathy based on network pharmacology: Role of ICA in DCM.; Authors - Liping Zhang
mouse	WB,IF	Oncotarget; Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro.; Authors - Endong Zhu
rat	WB	Pain Res Manag; Exploring the Analgesic Initiation Mechanism of Tuina in the Dorsal Root Ganglion of Minor CCI Rats via the TRPV1/TRPA1-cGMP Pathway; Authors - Zhenjie Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Venkata (Verified Customer) (01-03-2022)

I have loaded 50 and 100ug of protein in each of the lanes and the samples are from mouse heart and skeletal muscle tissues. I don't see expected bands in all the samples and most of the bands are non specific.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse cardiac and skeletal muscle

Beta Sarcoglycan Antibody Western Blot validation (1:500 dilution) in Mouse cardiac and skeletal muscle (Cat no:11936-1-AP)