SGEF Polyclonal antibody, PBS Only

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• SGEF Polyclonal antibody, PBS Only (12493-1-PBS)

• 

  WB analysis of PC-3 using 12493-1-AP (same clone as 12493-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12493-1-AP (same clone as 12493-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12493-1-AP (same clone as 12493-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 12493-1-AP (same clone as 12493-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12493-1-AP (same clone as 12493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12493-1-AP (same clone as 12493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

SGEF Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12493-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ARHGEF26, HMFN1864, Rho guanine nucleotide exchange factor 26, SH3 domain-containing guanine exchange factor

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Product Information

12493-1-PBS targets SGEF in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3198

  Product name: Recombinant human SGEF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-301 aa of BC016628

  Sequence: MISFLILPMQRVTRLPLLMDTICQKTPKDSPKYEVCKRALKEVSKLVRLCNEGARKMERTEMMYTINSQLEFKIKPFPLVSSSRWLVKRGELTAYVEDTVLFSRRTSKQQVYFFLFKDVLIITKKKSEESYNVNDYSLRDQLLVESCDNEELNSSPGKNSSTMLYSRQSSASHLFTLTVLSNHANEKVEMLLGAETQSERARWITALGHSSGKPPADRTSLTQVEIVRSFTAKQPDELSLQVADVVLIYQRVSDGWYEGERLRDGERGWFPMECAKEITCQATIDKNVERMGRLLGLETNV

• Full Name: Src homology 3 domain-containing guanine nucleotide exchange factor
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC016628
• Gene Symbol: SGEF
• Gene ID (NCBI): 26084
• RRID: AB_10896997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96DR7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.