SGK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SGK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11185-1-AP

Synonyms

H SGK2, SGK2

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Validation Data Gallery

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  WB analysis of human placenta using 11185-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11185-1-AP (SGK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11185-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11185-1-AP (SGK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11185-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11185-1-AP (SGK2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11185-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11185-1-AP (SGK2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse pancreas tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

11185-1-AP targets SGK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGK2 fusion protein Ag1669
• Full Name: serum/glucocorticoid regulated kinase 2
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC014037
• Gene Symbol: SGK2
• Gene ID (NCBI): 10110
• RRID: AB_2188525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Autophagy; Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits.; Authors - Seonghee Jung ; KO Validated