SGSM1 Polyclonal antibody

SGSM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21395-1-AP

Synonyms

KIAA1941, RUTBC2, SGSM1

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Validation Data Gallery

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  WB analysis of mouse testis using 21395-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21395-1-AP (SGSM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21395-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21395-1-AP (SGSM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skin using 21395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 21395-1-AP (SGSM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 21395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 21395-1-AP (SGSM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21395-1-AP is SGSM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGSM1 fusion protein Ag16070
• Full Name: small G protein signaling modulator 1
• Calculated Molecular Weight: 1148 aa, 130 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC111698
• Gene Symbol: SGSM1
• Gene ID (NCBI): 129049
• RRID: AB_10859829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.