SH2B1 Polyclonal antibody

SH2B1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12226-1-AP

Synonyms

KIAA1299, PSM, SH2B, SH2B adapter protein 1, SH2B adaptor protein 1, SH2B1

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Validation Data Gallery

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  WB analysis of HEK-293 using 12226-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12226-1-AP (SH2B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12226-1-AP

  IP result of anti-SH2B1 (IP:12226-1-AP, 4ug; Detection:12226-1-AP 1:600) with HEK-293 cells lysate 3000ug.

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  IHC staining of human ovary using 12226-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 12226-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 12226-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 12226-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12226-1-AP

  Immunofluorescent analysis of HeLa cells using 12226-1-AP (SH2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

12226-1-AP targets SH2B1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH2B1 fusion protein Ag2863
• Full Name: SH2B adaptor protein 1
• Calculated Molecular Weight: 426 aa, 45 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC010704
• Gene Symbol: SH2B1
• Gene ID (NCBI): 25970
• RRID: AB_2877838
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH2B1 is a member of the SH2-domain containing mediators family. SH2B1 has been shown to interact with INS receptor, Grb2, TrkA and Janus kinase 2. Variations close to or in the SH2B1 gene have been found to associate with obesity in two large genome wide association studies of body mass index (PMID: 19079260). SH2B1deletions are associated with severe early-onset obesity (PMID: 19966786).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,CoIP	Redox Biol; Neuronal SH2B1 attenuates apoptosis in an MPTP mouse model of Parkinson's disease via promoting PLIN4 degradation.; Authors - Xiaojuan Han