SH2D4A Polyclonal antibody

SH2D4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15957-1-AP

Synonyms

SH2A, SH2 domain-containing protein 4A, Protein phosphatase 1 regulatory subunit 38, PPP1R38

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Validation Data Gallery

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  WB analysis of HeLa using 15957-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15957-1-AP (SH2D4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15957-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15957-1-AP (SH2D4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15957-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15957-1-AP (SH2D4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15957-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15957-1-AP (SH2D4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

15957-1-AP targets SH2D4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH2D4A fusion protein Ag8713
• Full Name: SH2 domain containing 4A
• Calculated Molecular Weight: 454 aa, 53 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC014525
• Gene Symbol: SH2D4A
• Gene ID (NCBI): 63898
• RRID: AB_2286025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Aging (Albany NY); Integrated clustering signature of genomic heterogeneity, stemness and tumor microenvironment predicts glioma prognosis and immunotherapy response; Authors - Yangyang Wu ; KD Validated
human	IHC,WB	Cell Signal; SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway; Authors - Haoming Shi