SH3BGR Polyclonal antibody

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• SH3BGR Polyclonal antibody (21161-1-AP)

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SH3BGR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21161-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA
For FC, select conjugated version.

Synonyms

21-glutamic acid-rich protein, SH3 domain binding glutamic acid rich protein, SH3 domain-binding glutamic acid-rich protein, SH3BGR protein

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  WB analysis of mouse heart using 21161-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21161-1-AP (SH3BGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 21161-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21161-1-AP (SH3BGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 21161-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 21161-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 21161-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 21161-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 21161-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3BGR antibody (21161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21161-1-AP targets SH3BGR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14739

  Product name: Recombinant human SH3BGR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-239 aa of BC006371

  Sequence: MPLLLLGETEPLKLERDCRSPVEPWAAASPDLALACLCHCQDLSSGAFPNRGVLGGVLFPTVEMVIKVFVATSSGSIAIRKKQQEVVGFLEANKIDFKELDIAGDEDNRRWMRENVPGEKKPQNGIPLPPQIFNEEQYCGDFDSFFSAKEENIIYSFLGLAPPPDSKGSEKAEEGGETEAQKEGSEDVGNLPEAQEKNEEEGETATEETEEIAMEGAEGEAEEEEETAEGEEPGEDEDS

• Full Name: SH3 domain binding glutamic acid-rich protein
• Calculated Molecular Weight: 239 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC006371
• Gene Symbol: SH3BGR
• Gene ID (NCBI): 6450
• RRID: AB_2878820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55822
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The SH3BGR Polyclonal antibody (Cat# 21161-1-AP) has 1 citation. It was used in a study published in International Journal of Molecular Sciences (2021), which investigated how SH3-Binding Glutamic Acid Rich (SH3BGR) deficiency augments apoptosis in neonatal rat cardiomyocytes. The research falls within the fields of cardiac biology and programmed cell death. The antibody was applied in Western blot and immunofluorescence assays on human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Int J Mol Sci; SH3-Binding Glutamic Acid Rich-Deficiency Augments Apoptosis in Neonatal Rat Cardiomyocytes.; Authors - Anushka Deshpande ; KD Validated