SH3BGR Polyclonal antibody

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  WB analysis of mouse heart using 21161-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21161-1-AP (SH3BGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 21161-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21161-1-AP (SH3BGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21161-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21161-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 21161-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21161-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21161-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3BGR antibody (21161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

SH3BGR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21161-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

21 GARP, 21 glutamic acid rich protein, SH3BGR, SH3BGR protein

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

21161-1-AP targets SH3BGR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH3BGR fusion protein Ag14739
• Full Name: SH3 domain binding glutamic acid-rich protein
• Calculated Molecular Weight: 239 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC006371
• Gene Symbol: SH3BGR
• Gene ID (NCBI): 6450
• RRID: AB_2878820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55822
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Int J Mol Sci; SH3-Binding Glutamic Acid Rich-Deficiency Augments Apoptosis in Neonatal Rat Cardiomyocytes.; Authors - Anushka Deshpande ; KD Validated