SH3BGRL Polyclonal antibody, PBS Only

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• SH3BGRL Polyclonal antibody, PBS Only (11253-1-PBS)

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  WB analysis of mouse colon using 11253-1-AP (same clone as 11253-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11253-1-AP (SH3BGRL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11253-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse uterus using 11253-1-AP (same clone as 11253-1-PBS)

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 11253-1-AP (SH3BGRL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11253-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 11253-1-AP (same clone as 11253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11253-1-AP (SH3BGRL antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11253-1-PBS in a different storage buffer formulation.

SH3BGRL Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 11253-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

11253-1-PBS targets SH3BGRL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1780

  Product name: Recombinant human SH3BGRL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-114 aa of BC016709

  Sequence: MVIRVYIASSSGSTAIKKKQQDVLGFLEANKIGFEEKDIAANEENRKWMRENVPENSRPATGYPLPPQIFNESQYRGDYDAFFEARENNAVYAFLGLTAPPGSKEAEVQAKQQA

• Full Name: SH3 domain binding glutamic acid-rich protein like
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC016709
• Gene Symbol: SH3BGRL
• Gene ID (NCBI): 6451
• RRID: AB_3085369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75368
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SH3BGRL, an adaptor protein, is one member of the SH3BGR family. SH3BGRL is ubiquitously expressed in varied human tissues and organs, including the bone marrow, heart, lung, liver, and kidney. SH3BGRL encodes a small protein with a conserved proline-rich PLPPQIF region and two motifs, Homer EVH1-binding and SH3-binding motifs. As a scaffold protein, SH3BGRL could participate in the protein-protein interaction for the integration or/and crosstalk of signal transduction, membrane trafficking, cytoskeletal rearrangements, and other key cellular processes.