SH3D19 Polyclonal antibody

SH3D19 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21499-1-AP

Synonyms

ADAM binding protein Eve 1, EBP, EEN binding protein, EVE1, Kryn, SH3 domain containing 19, SH3D19, SH3P19



Tested Applications

Positive WB detected inHT-1080 cells, Y79 cells
Positive IHC detected inhuman heart, tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21499-1-AP is SH3D19 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH3D19 fusion protein Ag15915
Full Name SH3 domain containing 19
Calculated molecular weight 790 aa, 87 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC108890
Gene symbol SH3D19
Gene ID (NCBI) 152503
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SH3D19 antibody 21499-1-APDownload protocol
IHC protocol for SH3D19 antibody 21499-1-APDownload protocol
IF protocol for SH3D19 antibody 21499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols