Endophilin 1 Polyclonal antibody

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  WB analysis using 12435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12435-1-AP (SH3GL2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12435-1-AP

  IP result of anti-Endophilin 1 (IP:12435-1-AP, 4ug; Detection:12435-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 12435-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12435-1-AP (SH3GL2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12435-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12435-1-AP (SH3GL2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Endophilin 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12435-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

CNSA2, EEN B1, Endophilin 1, Endophilin A1, SH3 domain GRB2 like 2, SH3 domain protein 2A, SH3D2A, SH3GL2, SH3P4

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Tested Applications

• Positive WB detected in: C6 cells, mouse brain, rat brain
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

12435-1-AP targets Endophilin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3114

  Product name: Recombinant human SH3GL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-279 aa of BC032825

  Sequence: MSVAGLKKQFHKATQKVSEKVGGAEGTKLDDDFKEMERKVDVTSRAVMEIMTKTIEYLQPNPASRAKLSMINTMSKIRGQEKGPGYPQAEALLAEAMLKFGRELGDDCNFGPALGEVGEAMRELSEVKDSLDIEVKQNFIDPLQNLHDKDLREIQHHLKKLEGRRLDFDYKKERQGKIPDEELRQALEKFDESKEIAESSMFNLLEMDIEQVSQLSALVQAQLEYHKQAVQILQQVTVRLEERIRQASSQPRREYQPKPRMSLEFPTGDSTQPNGGLSH

• Full Name: SH3-domain GRB2-like 2
• Calculated Molecular Weight: 39.8 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC032825
• Gene Symbol: Endophilin 1
• Gene ID (NCBI): 6456
• RRID: AB_2187398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99962
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Neoplasia; Loss of Sh3gl2/Endophilin A1 Is a Common Event in Urothelial Carcinoma that Promotes Malignant Behavior.; Authors - Majumdar Shyama S ; KD Validated
human	WB	Invest Ophthalmol Vis Sci; Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma; Authors - Shuilian Chen