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  • KD/KO Validated

Endophilin 1 Polyclonal antibody

Endophilin 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12435-1-AP

Synonyms

CNSA2, EEN B1, Endophilin 1, Endophilin A1, SH3 domain GRB2 like 2, SH3 domain protein 2A, SH3D2A, SH3GL2, SH3P4



Tested Applications

Positive WB detected inC6 cells, mouse brain, rat brain
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12435-1-AP targets Endophilin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Endophilin 1 fusion protein Ag3114
Full Name SH3-domain GRB2-like 2
Calculated Molecular Weight 39.8 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC032825
Gene Symbol Endophilin 1
Gene ID (NCBI) 6456
RRIDAB_2187398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Endophilin 1 antibody 12435-1-APDownload protocol
IHC protocol for Endophilin 1 antibody 12435-1-APDownload protocol
IP protocol for Endophilin 1 antibody 12435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Neoplasia

Loss of Sh3gl2/Endophilin A1 Is a Common Event in Urothelial Carcinoma that Promotes Malignant Behavior.

Authors - Majumdar Shyama S
  • KD Validated
humanWB

Invest Ophthalmol Vis Sci

Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma

Authors - Shuilian Chen