SH3GL3 Polyclonal antibody

SH3GL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13849-1-AP

Synonyms

CNSA3, EEN 2B L3, EEN B2, Endophilin 3, Endophilin A3, HsT19371, SH3 domain GRB2 like 3, SH3 domain protein 2C, SH3D2C, SH3GL3, SH3P13

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Validation Data Gallery

• 

  WB analysis of human brain using 13849-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13849-1-AP (SH3GL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13849-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13849-1-AP (SH3GL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13849-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13849-1-AP (SH3GL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13849-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13849-1-AP (SH3GL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13849-1-AP targets SH3GL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH3GL3 fusion protein Ag4819
• Full Name: SH3-domain GRB2-like 3
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC042864
• Gene Symbol: SH3GL3
• Gene ID (NCBI): 6457
• RRID: AB_2187423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3GL3 is a novel member of SH3-containing family that has a src-homology-3 domain (SH3) at the C-terminus and drives protein-protein interactions through binding to proline-rich ligands. SH3GL3 is selectively expressed in brain and testis, and plays important role in signal transduction and regulation of synaptic vesicles.