Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18976-1-AP targets SH3PXD2A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | SH3 and PX domains 2A |
| Calculated Molecular Weight | 125 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | NM_014631 |
| Gene Symbol | SH3PXD2A |
| Gene ID (NCBI) | 9644 |
| RRID | AB_10598319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TCZ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SH3PXD2A is also named as FISH, KIAA0418, SH3MD1, TKS5. SH3PXD2A contains an amino-terminal PX domain followed by five SH3 domains. It is a cytoplasmic protein in normal fibroblasts (PMID: 19464300). The p140 and p130 forms of SH3PXD2A may be generated by other splice variations. The complexity of SH3PXD2A isoforms is also evident in different cell types. For example in human platelets a single band of 150 kDa was detected, whereas all three forms were found in human fibroblasts and vascular smooth muscle cells (PMID: 9687503). This antibody is specific to SH3PXD2A.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SH3PXD2A antibody 18976-1-AP | Download protocol |
| IHC protocol for SH3PXD2A antibody 18976-1-AP | Download protocol |
| WB protocol for SH3PXD2A antibody 18976-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Tks5 (SH3PXD2A), Cat# 18976-1-AP, has 15 total citations published in journals including Nature Communications, Autophagy, Circulation Research, Theranostics, Cell Death & Disease, Advanced Healthcare Materials, Cell Reports, Frontiers in Cell and Developmental Biology, Oncotarget, Journal of Oncology, Molecular Biology of the Cell, PLoS ONE, Prostate, and American Journal of Translational Research. Research fields span cancer invasion and metastasis, invadopodia and podosome biology, cell migration, and tumor–stroma interactions.
| Species | Application | Title |
|---|---|---|
Autophagy Starvation-inactivated MTOR triggers cell migration via a ULK1-SH3PXD2A/TKS5-MMP14 pathway in ovarian carcinoma | ||
Nat Commun Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling. | ||
Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. | ||
Theranostics IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis. | ||
Cell Death Dis Association of lncRNA SH3PXD2A-AS1 with preeclampsia and its function in invasion and migration of placental trophoblast cells. | ||
Adv Healthc Mater Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration |
