SH3RF1/POSH Polyclonal antibody

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• SH3RF1/POSH Polyclonal antibody (14649-1-AP)

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• KD/KO Validated

SH3RF1/POSH Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14649-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

POSH, SH3RF1, E3 ubiquitin-protein ligase SH3RF1, EC:2.3.2.27, KIAA1494

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  WB analysis of mouse brain using 14649-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (SH3RF1/POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of DU 145 using 14649-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (SH3RF1/POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 14649-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (SH3RF1/POSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 14649-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (SH3RF1/POSH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 14649-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (SH3RF1/POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 14649-1-AP

  IP result of anti-SH3RF1/POSH (IP:14649-1-AP, 4ug; Detection:14649-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14649-1-AP (SH3RF1/POSH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14649-1-AP (SH3RF1/POSH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, DU 145 cells, HEK-293 cells, human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, rat colon tissue, rat brain tissue, mouse brain tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14649-1-AP targets SH3RF1/POSH in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6243

  Product name: Recombinant human SH3RF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 696-1056 aa of BC053671

  Sequence: VTMVSPSTAGGPAQKLQGNGVAGSPSVVPAAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAAGLSPASVGLSHHSLASPQPAPLMPGSATHTAAISISRASAPLACAAAAPLTSPSITSASLEAEPSGRIVTVLPGLPTSPDSASSACGNSSATKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHGRAGSCPVDGDGPVTTAVAGAALAQDAFHRKASSLDSAVPIAPPPRQACSSLGPVLNESRPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI

• Full Name: SH3 domain containing ring finger 1
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 93-100 kDa
• GenBank Accession Number: BC053671
• Gene Symbol: SH3RF1/POSH
• Gene ID (NCBI): 57630
• RRID: AB_2187290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z6J0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.

Publications

What published studies show

Anti-POSH (SH3RF1) antibody, catalog #14649-1-AP, has 8 total citations published in journals including Cell Reports, Journal of Cell Biology, Human Molecular Genetics, International Journal of Biological Macromolecules, International Journal of Molecular Sciences, Expert Opinion on Therapeutic Targets, Scientific Reports, and iScience. Research fields span virology, synaptic biology, epilepsy, frontotemporal dementia, autism spectrum disorder, and diabetic cognitive dysfunction.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Biol Macromol; Snakehead vesiculovirus hijacks SH3RF1 for replication via mediating K63-linked ubiquitination at K264 of the phosphoprotein; Authors - Xiangmou Qin
mouse,human	WB,IP	Cell Rep; POSH regulates assembly of the NMDAR/PSD-95/Shank complex and synaptic function.; Authors - Minghui Yao
drosophila	IF	J Cell Biol; Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia.; Authors - Ryan J H West
mouse	WB	Int J Mol Sci; Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.; Authors - Sunil Goodwani
mouse,human	WB,IP,IF	Expert Opin Ther Targets; POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.; Authors - Xinshi Wang ; KD Validated
mouse	IF	Sci Rep; Identification of common biomarkers in diabetic kidney disease and cognitive dysfunction using machine learning algorithms; Authors - Jing Peng