SH3RF1/POSH Recombinant monoclonal antibody

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• SH3RF1/POSH Recombinant monoclonal antibody (88135-1-RR)

SH3RF1/POSH Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88135-1-RR

Clone No.260354A12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

POSH, SH3RF1, E3 ubiquitin-protein ligase SH3RF1, EC:2.3.2.27, KIAA1494

• 

  WB analysis using 88135-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88135-1-RR (SH3RF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 88135-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88135-1-RR (SH3RF1/POSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, DU 145 cells, A431 cells, Neuro-2a cells, C6 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88135-1-RR targets SH3RF1/POSH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6243

  Product name: Recombinant human SH3RF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 696-1056 aa of BC053671

  Sequence: VTMVSPSTAGGPAQKLQGNGVAGSPSVVPAAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAAGLSPASVGLSHHSLASPQPAPLMPGSATHTAAISISRASAPLACAAAAPLTSPSITSASLEAEPSGRIVTVLPGLPTSPDSASSACGNSSATKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHGRAGSCPVDGDGPVTTAVAGAALAQDAFHRKASSLDSAVPIAPPPRQACSSLGPVLNESRPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI

• Full Name: SH3 domain containing ring finger 1
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 93-100 kDa
• GenBank Accession Number: BC053671
• Gene Symbol: SH3RF1/POSH
• Gene ID (NCBI): 57630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z6J0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.