SH3RF3 Polyclonal antibody

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• SH3RF3 Polyclonal antibody (55373-1-AP)

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  IHC staining of human stomach using 55373-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55373-1-AP (SH3RF3 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 55373-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SH3RF3 antibody (55373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SH3RF3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 55373-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

POSH2, SH3MD4

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Tested Applications

• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55373-1-AP targets SH3RF3 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SH3 domain containing ring finger 3
• Calculated Molecular Weight: 93 kDa
• GenBank Accession Number: NM_001099289
• Gene Symbol: SH3RF3
• Gene ID (NCBI): 344558
• RRID: AB_3743206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TEJ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3RF3 is a ubiquitously expressed RING finger E3 ligase that belongs to the POSH protein family. SH3RF3 also has a partial CRIB domain that has RAC1-binding activity(PMID: 20696164). SH3RF3 contains four SH3 domains for protein interactions and a ring finger domain that confers E3 ubiquitin ligase activity, possibly targeting itself and other proteins for proteasomal degradation (PMID: 41291187).