SHARPIN Monoclonal antibody

SHARPIN Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3A9E2

Cat no : 67177-1-Ig

Synonyms

DKFZp434N1923, hSIPL1, SHARPIN, SIPL1

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Validation Data Gallery

• 

  WB analysis using 67177-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67177-1-Ig (SHARPIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67177-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67177-1-Ig (SHARPIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, A549 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67177-1-Ig targets SHARPIN in WB,ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SHARPIN fusion protein Ag6367
• Full Name: SHANK-associated RH domain interactor
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC025244
• Gene Symbol: SHARPIN
• Gene ID (NCBI): 81858
• RRID: AB_2882473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.