SHARPIN Monoclonal antibody

SHARPIN Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3A9E2

Cat no : 67177-1-Ig

Synonyms

DKFZp434N1923, hSIPL1, SHARPIN, SIPL1



Tested Applications

Positive WB detected inA431 cells, HeLa cells, A549 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67177-1-Ig targets SHARPIN in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SHARPIN fusion protein Ag6367
Full Name SHANK-associated RH domain interactor
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC025244
Gene symbol SHARPIN
Gene ID (NCBI) 81858
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHARPIN antibody 67177-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols