SHC4 Polyclonal antibody

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  WB analysis using 12641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12641-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12641-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12641-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 12641-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 12641-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12641-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12641-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 12641-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 12641-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

SHC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12641-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

SHC transforming protein 4, SH2 domain protein C4, RaLP, Rai-like protein, Rai like protein

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human brain tissue, HepG2 cells, human skeletal muscle tissue, mouse brain tissue, A375 cells, rat brain tissue
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

12641-1-AP targets SHC4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3336

  Product name: Recombinant human SHC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 324-630 aa of BC033907

  Sequence: NGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRGTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK

• Full Name: SHC (Src homology 2 domain containing) family, member 4
• Calculated Molecular Weight: 630 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC033907
• Gene Symbol: SHC4
• Gene ID (NCBI): 399694
• RRID: AB_10643243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6S5L8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Clin Sci (Lond); Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.; Authors - Zhiling Luo
human	IHC,IF	PeerJ; A new 12-gene diagnostic biomarker signature of melanoma revealed by integrated microarray analysis.; Authors - Liu Wanting W
mouse,human	WB,IHC,IF,CoIP	Cancer Lett; SHC4 orchestrates β-catenin pathway-mediated metastasis in triple-negative breast cancer by promoting Src kinase autophosphorylation; Authors - Wenjing Zhong ; KD Validated