SHC4 Polyclonal antibody

SHC4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

Publications

2

Cat no : 12641-1-AP

Synonyms

hShcD, Rai like protein, RaLP, SH2 domain protein C4, SHC transforming protein 4, SHC transforming protein D, SHC4, SHCD

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Validation Data Gallery

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  WB analysis using 12641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12641-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12641-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 12641-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12641-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human brain tissue, HepG2 cells, mouse brain tissue, human skeletal muscle tissue, A375 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12641-1-AP targets SHC4 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHC4 fusion protein Ag3336
• Full Name: SHC (Src homology 2 domain containing) family, member 4
• Calculated molecular weight: 630 aa, 69 kDa
• Observed molecular weight: 69 kDa
• GenBank accession number: BC033907
• Gene symbol: SHC4
• Gene ID (NCBI): 399694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Clin Sci (Lond); Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.; Authors - Zhiling Luo
human	IHC,IF	PeerJ; A new 12-gene diagnostic biomarker signature of melanoma revealed by integrated microarray analysis.; Authors - Liu Wanting W