SHD Polyclonal antibody

SHD Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20814-1-AP

Synonyms

SHD

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Validation Data Gallery

• 

  WB analysis of mouse brain using 20814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 20814-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20814-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 20814-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Neuro-2a using 20814-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed Neuro-2a cells using 20814-1-AP (SHD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Neuro-2a cells, NIH/3T3 cells, SMMC-7721 cells
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20814-1-AP is SHD Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHD fusion protein Ag14794
• Full Name: Src homology 2 domain containing transforming protein D
• Calculated Molecular Weight: 340 aa, 38 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC007206
• Gene Symbol: SHD
• Gene ID (NCBI): 56961
• RRID: AB_2878745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.