SHF Polyclonal antibody

SHF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21909-1-AP

Synonyms

SHF

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Validation Data Gallery

• 

  WB analysis using 21909-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21909-1-AP (SHF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21909-1-AP is SHF Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHF fusion protein Ag15083
• Full Name: Src homology 2 domain containing F
• Calculated Molecular Weight: 480 aa, 53 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC101601
• Gene Symbol: SHF
• Gene ID (NCBI): 90525
• RRID: AB_11182725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.