SHKBP1 Polyclonal antibody

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• SHKBP1 Polyclonal antibody (12703-1-AP)

SHKBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12703-1-AP Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PP203, SB1, SETA-binding protein 1, SH3KBP1 binding protein 1, SH3KBP1-binding protein 1

• 

  WB analysis of A431 using 12703-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12703-1-AP (SHKBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat stomach using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat stomach using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 12703-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12703-1-AP (SHKBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 12703-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SHKBP1 antibody (12703-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human stomach cancer tissue, human placenta tissue, mouse testis tissue, mouse brain tissue, rat stomach tissue, rat brain tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12703-1-AP targets SHKBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3386

  Product name: Recombinant human SHKBP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 358-707 aa of BC022855

  Sequence: MKDNDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGGVRVIVQHPETVGSGPQLFQTFTVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKILALESADGHGGCSAGNDIGPYGERDDQLVFIQKVVPSASQLFVRLSSTGQRVCSVRSVDGSPTTAFTVLECEGSRRLGSRPRRYLLTGQANGSLAMWDLTTAMDGLGQAPAGGLTEQELMEQLEHCELAPPAPSAPSWGCLPSPSPRISLTSLHSASSNTSLSGHRGSPSPPQAEARRRGGGSFVERCQELVRSGPDLRRPPTPAPWPSSGLGTPLTPPKMKLNETSF

• Full Name: SH3KBP1 binding protein 1
• Calculated Molecular Weight: 707 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC022855
• Gene Symbol: SHKBP1
• Gene ID (NCBI): 92799
• RRID: AB_10637860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TBC3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.