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  • KD/KO Validated

SHMT2 Polyclonal antibody

SHMT2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11099-1-AP

Synonyms

GLYA, Serine methylase, SHMT, SHMT2



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11099-1-AP targets SHMT2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHMT2 fusion protein Ag1584
Full Name serine hydroxymethyltransferase 2 (mitochondrial)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC011911
Gene symbol SHMT2
Gene ID (NCBI) 6472
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHMT2 antibody 11099-1-APDownload protocol
IHC protocol for SHMT2 antibody 11099-1-APDownload protocol
IF protocol for SHMT2 antibody 11099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC, WB

Am J Respir Cell Mol Biol

Inhibition of PHGDH Attenuates Bleomycin-induced Pulmonary Fibrosis.

Authors - Robert B Hamanaka
humanWB

J Biol Chem

De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.

Authors - Teresa W M Fan
mouseWB

Mol Genet Metab

Derangement of hepatic polyamine, folate, and methionine cycle metabolism in cystathionine beta-synthase-deficient homocystinuria in the presence and absence of treatment: Possible implications for pathogenesis.

Authors - Kenneth N Maclean
humanWB,IHC

J Biol Chem

TGF-β Promotes de novo Serine Synthesis for Collagen Production.

Authors - Recep Nigdelioglu
humanWB,IF

J Cell Sci

Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.

Authors - S John Calise
  • KD Validated
humanWB

Sci Rep

Vascular endothelial growth factor signaling requires glycine to promote angiogenesis.

Authors - Dongqing Guo