SHMT2 Polyclonal antibody

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  WB analysis using 11099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11099-1-AP

  WB result of SHMT2 antibody (11099-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHMT2 transfected HepG2 cells.

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  WB analysis of mouse kidney using 11099-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse brain using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11099-1-AP (SHMT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11099-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHMT2 antibody (11099-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 11099-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SHMT2 antibody (11099-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (red).

• Featured Product
• KD/KO Validated

SHMT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11099-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.1.2.1, SHMT

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, mouse kidney tissue, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: mouse brain tissue, human cervical cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

11099-1-AP targets SHMT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1584

  Product name: Recombinant human SHMT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 205-504 aa of BC011911

  Sequence: DYNQLALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH

• Full Name: serine hydroxymethyltransferase 2 (mitochondrial)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC011911
• Gene Symbol: SHMT2
• Gene ID (NCBI): 6472
• RRID: AB_2188452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P34897
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
human	IHC	EMBO Mol Med; Proteomics and personalized PDX models identify treatment for a progressive malignancy within an actionable timeframe; Authors - Georgina D Barnabas
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB,IHC	Am J Respir Cell Mol Biol; Inhibition of PHGDH Attenuates Bleomycin-induced Pulmonary Fibrosis.; Authors - Robert B Hamanaka
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu ; KD Validated