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  • KD/KO Validated

SHMT2 Polyclonal antibody

SHMT2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11099-1-AP

Synonyms

GLYA, Serine methylase, SHMT, SHMT2



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11099-1-AP targets SHMT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHMT2 fusion protein Ag1584
Full Name serine hydroxymethyltransferase 2 (mitochondrial)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC011911
Gene symbol SHMT2
Gene ID (NCBI) 6472
RRIDAB_2188452
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SHMT2 antibody 11099-1-APDownload protocol
IHC protocol for SHMT2 antibody 11099-1-APDownload protocol
IF protocol for SHMT2 antibody 11099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Pineal Res

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Authors - Li Cui
humanWB

Nat Commun

Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.

Authors - Eduardo Balsa
humanWB

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
mouseIHC, WB

Am J Respir Cell Mol Biol

Inhibition of PHGDH Attenuates Bleomycin-induced Pulmonary Fibrosis.

Authors - Robert B Hamanaka
humanWB

PLoS Pathog

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Authors - Muyu Xu
  • KD Validated
humanWB,IHC

Genomics

SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.

Authors - Fu-Chun Huo