SHMT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SHMT2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 11099-1-AP

Synonyms

GLYA, Serine methylase, SHMT, SHMT2

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Validation Data Gallery

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  WB analysis using 11099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11099-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 11099-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: human cervical cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

11099-1-AP targets SHMT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHMT2 fusion protein Ag1584
• Full Name: serine hydroxymethyltransferase 2 (mitochondrial)
• Calculated molecular weight: 55 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC011911
• Gene symbol: SHMT2
• Gene ID (NCBI): 6472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu ; KD Validated
human	WB,IHC	Genomics; SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.; Authors - Fu-Chun Huo
human	WB	Am J Respir Cell Mol Biol; TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-dependent ATF4 Activation.; Authors - Erin M O'Leary