Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
|Positive IHC detected in
|human cervical cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11099-1-AP targets SHMT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|SHMT2 fusion protein Ag1584
|serine hydroxymethyltransferase 2 (mitochondrial)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
J Pineal Res
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.
cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.
Am J Respir Cell Mol Biol
Inhibition of PHGDH Attenuates Bleomycin-induced Pulmonary Fibrosis.
SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.
SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.