SHMT2 Polyclonal antibody

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SHMT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 11099-1-AP Copied!

Publications (42)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

EC:2.1.2.1, SHMT

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  WB analysis using 11099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HepG2 using 11099-1-AP

  WB result of SHMT2 antibody (11099-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHMT2 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 11099-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11099-1-AP (SHMT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11099-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHMT2 antibody (11099-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11099-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SHMT2 antibody (11099-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of MCF-7 using 11099-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SHMT2 Polyclonal antibody (11099-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, mouse kidney tissue, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: mouse brain tissue, human cervical cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11099-1-AP targets SHMT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1584

  Product name: Recombinant human SHMT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 205-504 aa of BC011911

  Sequence: DYNQLALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH

• Full Name: serine hydroxymethyltransferase 2 (mitochondrial)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC011911
• Gene Symbol: SHMT2
• Gene ID (NCBI): 6472
• RRID: AB_2188452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P34897
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

SHMT2 antibody (Cat# 11099-1-AP) has 42 citations across journals including Nature Communications, Cell Research, Cancer Research, Molecular Cell, Advanced Science, Cell Reports, Journal of Biological Chemistry, FASEB Journal, and others. Research fields span cancer metabolism (renal cell carcinoma, breast cancer, gastric cancer, bladder cancer), serine/glycine one-carbon metabolism, mitochondrial dysfunction, pulmonary fibrosis, neurodegeneration, and metabolic reprogramming in disease contexts.

Automatically generated from the 42 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	WB	Cancer Res; De Novo Serine Synthesis is a Metabolic Vulnerability that can be Exploited to Overcome Sunitinib Resistance in Advanced Renal Cell Carcinoma; Authors - Manon Teisseire
		Nat Commun; Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects; Authors - Ritobrata Ghose
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
human	WB,IF	Nat Commun; Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.; Authors - S. Kourtis
human	WB	Nat Commun; Complex I protein NDUFB9 is a metabolic vulnerability in triple negative breast cancer brain metastases.; Authors - Mingxi Lin