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  • KD/KO Validated

SHMT2 Polyclonal antibody

SHMT2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11099-1-AP

Synonyms

GLYA, Serine methylase, SHMT, SHMT2



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11099-1-AP targets SHMT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHMT2 fusion protein Ag1584
Full Name serine hydroxymethyltransferase 2 (mitochondrial)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC011911
Gene symbol SHMT2
Gene ID (NCBI) 6472
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SHMT2 antibody 11099-1-APDownload protocol
IHC protocol for SHMT2 antibody 11099-1-APDownload protocol
IF protocol for SHMT2 antibody 11099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Pineal Res

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Authors - Li Cui
humanWB

Nat Commun

Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.

Authors - Eduardo Balsa
humanWB

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
humanWB

PLoS Pathog

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Authors - Muyu Xu
  • KD Validated
humanWB,IHC

Genomics

SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.

Authors - Fu-Chun Huo
humanWB

Am J Respir Cell Mol Biol

TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-dependent ATF4 Activation.

Authors - Erin M O'Leary