SHOX2 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• SHOX2 Polyclonal antibody (16366-1-AP)

• Featured Product
• KD/KO Validated

SHOX2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16366-1-AP Copied!

Publications (4)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SHOT, Paired-related homeobox protein SHOT, OGI2X, OG12X, OG12

• 

  WB analysis using 16366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16366-1-AP (SHOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 16366-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16366-1-AP (SHOX2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 16366-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16366-1-AP (SHOX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 16366-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16366-1-AP (SHOX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SHOX2 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: MCF-7 cells, Raji cells, PC-12 cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16366-1-AP targets SHOX2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9156

  Product name: Recombinant human SHOX2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-355 aa of BC008829

  Sequence: MEELTAFVSKSFDQKVKEKKEAITYREVLESGPLRGAKEPTGCTEAGRDDRSSPAVRAAGGGGGGGGGGGGGGGGGGVGGGGAGGGAGGGRSPVRELDMGAAERSREPGSPRLTEGRRKPTKAEVQATLLLPGEAFRFLVSPELKDRKEDAKGMEDEGQTKIKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQENQLHKGVLIGAASQFEACRVAPYVNVGALRMPFQQDSHCNVTPLSFQVQAQLQLDSAVAHAHHHLHPHLAAHAPYMMFPAPPFGLPLATLAADSASAASVVAAAAAAKTTSKNSSIADLRLKAKKHAAALGL

• Full Name: short stature homeobox 2
• Calculated Molecular Weight: 355 aa, 38 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC008829
• Gene Symbol: SHOX2
• Gene ID (NCBI): 6474
• RRID: AB_2918046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60902
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The SHOX2 Polyclonal antibody (Cat# 16366-1-AP) has 4 citations published in Oxidative Medicine and Cellular Longevity, Drug Development Research, Frontiers in Genetics, and Aging (Albany NY). The associated research fields include cardiovascular biology (heart rate regulation and sinoatrial node dysfunction), gastric cancer (Wnt/β-catenin signaling), and glioma (hypoxia-driven gene signatures and drug resistance). All citations used the antibody for Western blot in human or mouse samples.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; NRF-2/HO-1 Pathway-Mediated SHOX2 Activation Is a Key Switch for Heart Rate Acceleration by Yixin-Fumai Granules; Authors - Heng Zhang ; KD Validated
human	WB	Drug Dev Res; Downregulation of short-stature homeobox protein 2 suppresses gastric cancer cell growth and stemness in vitro and in vivo via inactivating wnt/β-catenin signaling; Authors - Xiangyu Chen
human	WB	Front Genet; A novel hypoxia-driven gene signature that can predict the prognosis and drug resistance of gliomas; Authors - Peng Ren ; KD Validated
mouse	WB	Aging (Albany NY); D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism; Authors - Heng Zhang