SHOX2 Polyclonal antibody

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  WB analysis using 16366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16366-1-AP (SHOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 16366-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16366-1-AP (SHOX2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 16366-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16366-1-AP (SHOX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 16366-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16366-1-AP (SHOX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SHOX2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16366-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SHOT, Paired-related homeobox protein SHOT, OGI2X, OG12X, OG12

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Tested Applications

• Positive WB detected in: MCF-7 cells, Raji cells, PC-12 cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below

Product Information

16366-1-AP targets SHOX2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9156

  Product name: Recombinant human SHOX2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-355 aa of BC008829

  Sequence: MEELTAFVSKSFDQKVKEKKEAITYREVLESGPLRGAKEPTGCTEAGRDDRSSPAVRAAGGGGGGGGGGGGGGGGGGVGGGGAGGGAGGGRSPVRELDMGAAERSREPGSPRLTEGRRKPTKAEVQATLLLPGEAFRFLVSPELKDRKEDAKGMEDEGQTKIKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQENQLHKGVLIGAASQFEACRVAPYVNVGALRMPFQQDSHCNVTPLSFQVQAQLQLDSAVAHAHHHLHPHLAAHAPYMMFPAPPFGLPLATLAADSASAASVVAAAAAAKTTSKNSSIADLRLKAKKHAAALGL

• Full Name: short stature homeobox 2
• Calculated Molecular Weight: 355 aa, 38 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC008829
• Gene Symbol: SHOX2
• Gene ID (NCBI): 6474
• RRID: AB_2918046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60902
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Genet; A novel hypoxia-driven gene signature that can predict the prognosis and drug resistance of gliomas; Authors - Peng Ren ; KD Validated
mouse	WB	Oxid Med Cell Longev; NRF-2/HO-1 Pathway-Mediated SHOX2 Activation Is a Key Switch for Heart Rate Acceleration by Yixin-Fumai Granules; Authors - Heng Zhang ; KD Validated
mouse	WB	Aging (Albany NY); D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism; Authors - Heng Zhang
human	WB	Drug Dev Res; Downregulation of short-stature homeobox protein 2 suppresses gastric cancer cell growth and stemness in vitro and in vivo via inactivating wnt/β-catenin signaling; Authors - Xiangyu Chen