SHPRH Polyclonal antibody

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  WB analysis of mouse testis using 21955-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21955-1-AP (SHPRH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 21955-1-AP

  IP result of anti-SHPRH (IP:21955-1-AP, 4ug; Detection:21955-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human colon using 21955-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21955-1-AP (SHPRH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21955-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21955-1-AP (SHPRH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SHPRH Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 21955-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

KIAA2023, SHPRH

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21955-1-AP targets SHPRH in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16635

  Product name: Recombinant human SHPRH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1334-1683 aa of BC117686

  Sequence: EYKLLHEYWMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAFATSQLQKKLGQLLYLTNLEKSQDKTSGGVNPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKANQEEDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKRDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAERSHTNSSAKHSEASVLTVADLADLFTKETEELE

• Full Name: SNF2 histone linker PHD RING helicase
• Calculated Molecular Weight: 1683 aa, 193 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC117686
• Gene Symbol: SHPRH
• Gene ID (NCBI): 257218
• RRID: AB_2878954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q149N8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.