SHROOM1 Polyclonal antibody

SHROOM1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 18218-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Apical protein 2, APXL2, KIAA1960, Protein Shroom1, shroom family member 1, SHROOM1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of MCF-7 using 18218-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 18218-1-AP (SHROOM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A375 using 18218-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 18218-1-AP (SHROOM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of MCF-7 using 18218-1-AP
IP result of anti-SHROOM1 (IP:18218-1-AP, 4ug; Detection:18218-1-AP 1:700) with MCF-7 cells lysate 3600 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	MCF7 cells, A375 cells
Positive IP detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18218-1-AP targets SHROOM1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12977 Product name: Recombinant human SHROOM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 503-852 aa of BC104914 Sequence: VPADALGLSGNDTPGPSHNTALARGTGQPGSRPTWPSQCLEELVQELARLDPSLCDPLASQPSPEPPLGLLDGLIPLAEVRAAMRPACGEAGEEAASTFEPGSYQFSFTQLLPAPREETRLENPATHPVLDQPCGQGLPAPNNSIQGKKVELAARLQKMLQDLHTEQERLQGEAQAWARRQAALEAAVRQACAPQELERFSRFMADLERVLGLLLLLGSRLARVRRALARAASDSDPDEQASLLQRLRLLQRQEEDAKELKEHVARRERAVREVLVRALPVEELRVYCALLAGKAAVLAQQRNLDERIRLLQDQLDAIRDDLGHHAPSPSPARPPGTCPPVQPPFPLLLT Predict reactive species
Full Name	shroom family member 1
Calculated Molecular Weight	852 aa, 91 kDa
Observed Molecular Weight	100-130 kDa
GenBank Accession Number	BC104914
Gene Symbol	SHROOM1
Gene ID (NCBI)	134549
RRID	AB_10858616
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q2M3G4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IP protocol for SHROOM1 antibody 18218-1-AP	Download protocol
WB protocol for SHROOM1 antibody 18218-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The SHROOM1 Polyclonal antibody (Cat# 18218-1-AP) has 1 total citation. It was cited in PLoS Pathogens in a study titled "Defining the proteolytic landscape during enterovirus infection" (2020), where it was used for Western blot analysis in human samples. The research field is virology, specifically enterovirus pathogenesis and host protease interactions.