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  • KD/KO Validated

SIAH2 Polyclonal antibody

SIAH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12651-1-AP

Synonyms

Siah 2, RING-type E3 ubiquitin transferase SIAH2, hSiah2, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH2



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12651-1-AP targets SIAH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIAH2 fusion protein Ag3342
Full Name seven in absentia homolog 2 (Drosophila)
Calculated Molecular Weight 324 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC013082
Gene Symbol SIAH2
Gene ID (NCBI) 6478
RRIDAB_2877868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian Seven-in-absentia homolog 2 (SIAH2), is a RING finger type ubiquitin ligase with a catalytic RING domain on its N-terminus, followed by two zinc fingers and a C-terminal substrate binding domain. Siah is a mammalian homolog of Sina, a Drosophila protein functioning in eye development. Mammals express two isoforms, Siah1 and 2, and mice exhibit two Siah1 isotypes, 1a and 1b. SIAH2 indirectly regulates the level of HIF-1α by promoting degradation of PHDs by UPS. SIAH2, in turn, is positively regulated by hypoxia-dependent transcriptional and posttranscriptional mechanisms.

Protocols

Product Specific Protocols
WB protocol for SIAH2 antibody 12651-1-APDownload protocol
IHC protocol for SIAH2 antibody 12651-1-APDownload protocol
IF protocol for SIAH2 antibody 12651-1-APDownload protocol
IP protocol for SIAH2 antibody 12651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
  • KD Validated
humanWB

Nucleic Acids Res

P-TEFb is degraded by Siah1/2 in quiescent cells.

Authors - Fang Huang
  • KD Validated
humanWB

J Exp Clin Cancer Res

PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer

Authors - Liang Xu

Cell Death Dis

PIWIL2 suppresses Siah2-mediated degradation of HDAC3 and facilitates CK2α-mediated HDAC3 phosphorylation.

Authors - Yingying Zhang
mouseIF,WB

Int Immunopharmacol

Methylene blue targets PHD3 expression in murine microglia to mitigate lipopolysaccharide-induced neuroinflammation and neurocognitive impairments

Authors - Guoyao Ou
humanWB,IP

Toxins (Basel)

Ochratoxin A Induces Steatosis via PPARγ-CD36 Axis.

Authors - Qian-Wen Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Patrick (Verified Customer) (11-13-2019)

Couldn't detect endogenous or overexpressed SIAH2

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: 293T